Indian Council of Agricultural Research (ICAR) Nucleic Acid Sequencing and Genotyping Service Tender India 2026

Bidders must submit GST registration, PAN, experience certificates, financial statements, and EMD documents as part of the bid. Provide detailed bioinformatics pipelines, Illumina platform credentials, and sample pickup logistics. Ensure compliance with ISI/ISO-quality controls and data privacy requirements, and confirm delivery milestones for 50-60 cattle samples.

Required documents include GST certificate, PAN, experience certificates in NGS, financial statements, EMD, technical bid detailing sequencing workflow, OEM authorizations if any, and data security commitments. Upload these with the technical bid to demonstrate capability for 10-15x coverage and QC thresholds.

The project requires Illumina paired-end reads of at least 150 bases, insert size 300-500 bp, and target depth 10-15x against the latest cow reference genome (NCBI/Ensembl). Data quality must meet 90% of bases at Q30 or higher; deliver cleaned FASTQ and indexed BAM/CRAM with variant annotations.

Approximately 50-60 whole blood samples in EDTA tubes will be picked up by the vendor from designated areas anywhere in India. Vendor bears responsibility for pickup arrangements and for any failed QC samples, which may be re-collected at no extra charge.

Deliverables include primary QC reports, cleaned FASTQ, aligned BAM/CRAM with index, variant calls (SNPs/INDELs) with annotations, per-sample summaries, and population-level allele frequencies plus GO and pathway enrichment results. All outputs must be suitable for downstream veterinary/genomic interpretation.

Bidders must demonstrate prior experience in high-throughput sequencing projects using Illumina platforms, with at least one cattle genome study or equivalent, and provide references. Also show capability to process 50+ samples with robust QC and downstream functional analysis.

Required standards include Q30 ≥ 90% for bases, DNA quality thresholds (A260/280 1.8-2.0, A260/230 ≥2.0), and use of validated bioinformatics pipelines for variant detection and annotation. Data should be delivered in standard formats with complete metadata.

Specific delivery timelines and payment terms are not provided in the data. Expect contract milestones aligned with sample pickup, sequencing completion, and data delivery; payment terms likely per government procurement norms and contract specifics to be finalized in the ATC/contract.