Open Tender for Protease and Phosphatase Inhibitor Cocktail,RIPA Radio Immunoprecipitation Assay Buffer,Blasticidin in MUMBAI, MAHARASHTRA

RIPA Radio Immunoprecipitation Assay Buffer Formulation must precisely contain 50 mM Tris HCl pH 7point 8 8point2 at 25 degree C 150 mM NaCl 1 dot 0percentage non ionic detergent IGEPAL CA 630 or exact NP 40 equivalent 0 dot 5percentage sodium deoxycholate and 0 dot 1percentage SDS dot Pack size 50 ml Detailed specifications as per attached annexure 1 dot

Broad Spectrum Phosphatase Inhibitor Cocktail Exactly one tablet should be sufficient to treat 10 mL of cell lysate or extraction buffer without requiring weighing or aliquoting Pack size 20 tablets per pack Detailed specifications as per attached annexure 1

Ponceau S Solution 0 dot 1 percentage w by v in 5 percentage Acetic Acid Staining action must be completely reversible allowing rapid destaining with water or standard wash buffers like TBST PBST without altering protein antigenicity or interfering with downstream immunological detection Pack size 1L Detailed specifications as per attached annexure 1

PVDF Transfer Membrane 0 dot 22 micro m pore size 8 dot 5 cm x 10 m roll Must be explicitly validated for high resolution Western blotting and be fully compatible with downstream protein sequencing applications eg N dash terminal degradation Detailed specifications as per attached annexure 1

PVDF Transfer Membrane for Fluorescent Western Blotting 0 dot 45 micro m nominal pore size 8 dot 5 cm x 10 m roll Hydrophobic Polyvinylidene fluoride PVDF membrane specifically optimized for quantitative fluorescent Western blotting applications Detailed specifications as per attached annexure 1